Inadequate Interleukin 2 Production
نویسندگان
چکیده
منابع مشابه
Defective Interleukin 2 Production in Tuberculosis
Some patients with active tuberculosis lack a delayed skin test reaction to PPD (1-4). The basis for this immunologic hyporesponsiveness has been investigated using in vitro systems. Tuberculin-induced blastogenic responses of lymphocytes are decreased in such patients compared with healthy tuberculin reactors (5, 6). This is not simply due to a dearth of responsive cells in the circulation. Ra...
متن کاملSodium azide enhancement of interleukin-2 production.
The production of large quantities of Interleukin-2 (IL-2) from normal human lymphocytes has been limited by the short production and release period, as well as its absorption by the responsive cell population. We report the utilization of sodium azide (NaN3, 0.01% final concentration) to allow continued production of IL-2 as long as 72-96 hr and thereby increase the yield significantly. Cell c...
متن کاملOptimization of Cloning Conditions for high-level Production of Recombinant Mouse Interleukin-2 in Escherichia coli
Backgrounds and objectives: Interleukin 2 (IL-2) secreted by activated CD4+ T cells has been known as a major mediator in both adaptive and native immune system due to a board range of effects on different cells in the immunity system (1-6). Methods: cDNA synthesis was performed using gene- specific primers designed by Gene Runner software after RNA extraction of mouse splenocytes. PCR pro...
متن کاملThe Effect of Mg2+ and Mn2+ on Over-Production of Interleukin-2 in Recombinant E.coli
In order to increase the productivity of human interleukin-2 (IL-2) as a model protein in the recombinant E.coliBL21 (DE3), the effect of magnesium acetate and manganese acetate was studied based on full factorial design experiments magnesium acetate and manganese acetate and their interaction exhibited an increasing effect on IL-2 expression level upto 25.24%, 16.80% and 23.30%, respective...
متن کاملProfile of interleukin-2 production in human mononuclear cells and T-cell lines
Introduction: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role Background and Objective: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role in activation, growth and differentiation of several immune cells. Moreover IL-2 is known as a pro-inflammatory and anti-tumoral cytokine. In addition deysregulation of IL-2 in some diseases such as ...
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ژورنال
عنوان ژورنال: Annals of Surgery
سال: 1984
ISSN: 0003-4932
DOI: 10.1097/00000658-198409000-00008